Kayan Gwaji Mai Sauri na IgG/IgM na Cutar Lyme (Gwajin Immunochromatographic)
Cikakkun bayanai game da samfurin
Amfani da aka yi niyya
Wannan samfurin ya dace da gwajin jini na jini/plasma/jini gaba ɗaya don gano ƙwayoyin rigakafi daga cutar Lyme. Gwaji ne mai sauƙi, mai sauri kuma ba na kayan aiki ba.
Ka'idar Gwaji
Wannan gwajin rigakafi ne na gefe. Kaset ɗin gwajin ya ƙunshi: 1) wani kushin haɗin gwiwa mai launin burgundy wanda ke ɗauke da antigen mai hade da juna wanda aka haɗa shi da colloid gold da zomo IgG-gold conjugates, 2) wani tsiri na membrane na nitrocellulose wanda ke ɗauke da madaukai biyu na gwaji (madaukai M da G) da kuma madaukai na sarrafawa (madaukai C).
Babban Abubuwan da ke Ciki
An jera abubuwan da aka bayar a cikin tebur.
| Kayan aiki / an bayar; | Adadi (Kit/Gwaji 1) | Adadi (Gwaje-gwaje 5/Kit) | Adadi (Gwaje-gwaje 25/Kit) |
| Kayan Gwaji | Gwaji 1 | Gwaje-gwaje 5 | Gwaje-gwaje 25 |
| Buffer | Kwalba 1 | Kwalabe 5 | Kwalabe 25/2 |
| Mai sauke dropper | Guda 1 | Kwamfuta 5 | Kwamfuta 25 |
| Jakar Sufuri ta Samfura | Guda 1 | Kwamfuta 5 | Kwamfuta 25 |
| Lancet da za a iya zubarwa | Guda 1 | Kwamfuta 5 | Kwamfuta 25 |
| Umarnin Amfani | Guda 1 | Guda 1 | Guda 1 |
| Takardar Shaidar Daidaito | Guda 1 | Guda 1 | Guda 1 |
Gudun Aiki
Mataki na 1: Yin samfur
A tattara jinin ɗan adam/jini/jini gaba ɗaya yadda ya kamata.
Mataki na 2: Gwaji
(1) Cire bututun cirewa daga kayan aikin da akwatin gwaji daga jakar fim ɗin ta hanyar yage ramin. Sanya su a kan layin kwance. Buɗe jakar foil ɗin aluminum na katin dubawa. Cire katin gwaji a ajiye shi a kwance a kan teburi.
(2) Yi amfani da pipette mai yarwa, a zuba jinin 4μL na jini (ko plasma), ko kuma jinin 4μL gaba ɗaya a cikin rijiyar samfurin da ke kan kaset ɗin gwaji.
(3) Buɗe bututun buffer ta hanyar karkatar da saman. Sanya digo 3 (kimanin 80 μL) na mai narkar da gwaji a cikin mai narkar da gwajin mai siffar zagaye. Ƙidaya kaɗan.
Mataki na 3: Karatu
A karanta sakamakon a minti 10-15. Sakamakon bayan minti 20 ba shi da inganci.
Fassarar Sakamako
Sakamako mara kyau
Layin sarrafa inganci C ne kawai ke bayyana kuma layukan ganowa G da M ba sa nunawa, yana nufin cewa ba a gano wani maganin rigakafi ba kuma sakamakon ya kasance mara kyau.
Sakamako Mai Kyau
Idan layin kula da inganci na C da layin gano M suka bayyana = an gano maganin rigakafi na IgM na cutar Lyme, kuma sakamakon ya nuna cewa maganin rigakafi na IgM yana da kyau.
Idan layin kula da inganci na C da layin gano G sun bayyana = an gano maganin rigakafi na cutar Lyme kuma sakamakon ya nuna yana da kyau ga maganin rigakafi na IgG.
Idan layin kula da inganci na C da layin gano G da M sun bayyana = an gano ƙwayoyin rigakafi na IgG da IgM na cutar Lyme, kuma sakamakon ya nuna cewa suna da kyau ga ƙwayoyin rigakafi na IgG da IgM.
Sakamako mara inganci
Idan ba za a iya lura da layin kula da inganci na C ba, sakamakon ba zai yi daidai ba ko da kuwa layin gwaji ya nuna, kuma ya kamata a maimaita gwajin.
Bayanin Oda
| Sunan Samfuri | Kyanwa. A'a | Girman | samfurin | Rayuwar shiryayye | Canja wurin Wucin Gadi & Tsaye. Temp. |
| Kayan Gwaji Mai Sauri na IgG/IgM na Cutar Lyme (Gwajin Immunochromatographic) | B026CH-01 | Gwaji/kit 1 | S/P/WB | Watanni 18 | 2-30℃ / 36-86℉ |
| B026CH-05 | Gwaje-gwaje 5/kayan aiki | ||||
| B026CH-25 | Gwaje-gwaje 25/kayan aiki |
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