Kwayar cuta ta Monkeypox Ainihin Kayan Aikin PCR da Aka Fitar da Hoton

Kwayar cutar Monkeypox na Real Time PCR Kit

Takaitaccen Bayani:

Cat.A'a	Saukewa: B001P-01	Girman	Gwaje-gwaje 48/Kit
Misali	Serum/ Lesion Exudate	Trans.& Sto.Temp.	-25 ～ -15 ℃
Tsarin	Premix an lyophilized kuma an raba shi a cikin tubes PCR 8 tsiri
Abubuwan da ake Aiwatar da su	Kayan aikin PCR na ainihi kamar ABI7500, Bio-Rad CFX96, SLAN-96S, QuantStudio da BTK-8

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Cikakken Bayani

Tags samfurin

Bayanin samfur

Amfani da Niyya

Ana amfani da shi don gano ƙwayar cuta ta Monkeypox a cikin jinin ɗan adam ko lahani exudate samfurori ta amfani da tsarin PCR na ainihi.

Ƙa'idar Gwaji

Wannan samfurin tsarin bincike ne na Taqman® na ainihin lokaci na PCR.An ƙera ƙayyadaddun ƙayyadaddun bayanai da bincike don gano ƙwayar cuta ta Monkeypox F3L.Ikon cikin gida da ke niyya ga asalin halittar ɗan adam yana lura da tarin samfurin, sarrafa samfurin da tsarin PCR na ainihi don guje wa sakamako mara kyau.Kit ɗin shine cikakken tsarin lyophilized premix, wanda ya haɗa da kayan da ake buƙata don gano ƙwayar cuta ta Monkeypox: nucleic acid amplification enzyme, UDG enzyme, maida martani, ƙayyadaddun firamare da bincike.

Babban Abubuwan Ciki

Abubuwan da aka bayar an jera su a cikin tebur.

Abubuwan da aka gyara	Kunshin	Abun ciki
Kwayar cuta ta MonkeypoxLyophilized Premix	8 tubes PCR× 6 jaka	Abubuwan farko, bincike, dNTP/dUTP Mix, Mg2+, Taq DNA polymerase, UDG Enzyme
MPV Kyakkyawan Sarrafa	400 μL × 1 tube	Jerin DNA mai ɗauke da kwayoyin Target
MPV Korau Control	400 μL × 1 tube	Jerin DNA mai ɗauke da sashin kwayoyin halittar ɗan adam
Magani na Rushewa	1 ml × 1 tube	Stabilizer
Certificate of Conformity	guda 1	/

Gudun Ayyuka

1. MisaliTarin:Ya kamata a tattara samfurori a cikin bututu mara kyau daidai

tare da daidaitattun ƙayyadaddun fasaha.

2. Reagent Preparation (Yankin Shirye-shiryen Reagent)

Fitar da abubuwan da ke cikin kit ɗin, daidaita su a zafin daki don amfanin jiran aiki.

3. Sarrafa Samfura (Yankin Sarrafa Samfura)

3.1 Nucleic acid hakar

Ana ba da shawarar ɗaukar samfuran ruwa na 200μL, Kulawa mai Kyau da Kulawa mara kyau don hakar acid nucleic, bisa ga daidaitattun buƙatu da hanyoyin abubuwan cirewar DNA na hoto ko bidiyo mai zagaya yanar gizo da sauri.

3.2 Lyophilized foda dissolving da samfur ƙari

Shirya ƙwayar cuta ta Monkeypox Lyophilized premix bisa ga adadin samfuran.Samfurin ɗaya yana buƙatar bututun PCR guda ɗaya mai ɗauke da Lyophilized premix foda.Ya kamata a kula da iko mara kyau & ingantaccen iko azaman samfuri biyu.

(1) Ƙara Magani na Dissolving 15μL a cikin kowane PCR bututu dauke da Lyophilized premix, sa'an nan ƙara 5μL fitar da samfurori / Korau Control / Kyakkyawan Control a cikin kowane PCR tube bi da bi.

(2) Rufe bututun PCR damtse, jujjuya bututun PCR da hannu har sai an narkar da foda na lyophilized gaba daya kuma a hade, tattara ruwan zuwa kasan bututun PCR ta hanyar rage saurin gudu nan take.

(3) Idan kayi amfani da kayan aikin PCR na yau da kullun don ganowa, to kai tsaye canja wurin bututun PCR zuwa yankin haɓakawa;Idan amfani da BTK-8 don ganowa, to, kuna buƙatar aiwatar da ayyuka masu zuwa: canja wurin ruwa na 10 μL daga bututun PCR zuwa guntun amsawar BTK-8.Bututun PCR ɗaya yayi daidai da rijiya ɗaya akan guntu.A lokacin aikin bututun, tabbatar da cewa pipette yana tsaye 90 digiri.Dole ne a sanya tukwici mai shinge na aerosol a tsakiyar rijiyar tare da matsakaicin ƙarfi kuma a daina tura pipette lokacin da ya isa na farko (don guje wa kumfa).Bayan an cika rijiyoyin, a fitar da membrane na amsawa don rufe duk rijiyoyin sannan a tura guntu zuwa wurin gano haɓakawa.

4. PCR Amplification (Yankin Ganewa)

4.1 Saka PCR tubes/reaction guntu a cikin tankin amsawa kuma saita sunayen kowane amsa da kyau a cikin daidaitaccen tsari.

4.2 Saitunan gano haske mai haske: (1) Kwayar cuta ta Monkeypox (FAM);(2) Kula da Ciki (CY5).

4.3 Gudun ƙa'idar keke mai zuwa

Yarjejeniyar ABI7500, Bio-Rad CFX96, SLAN-96S, QuantStudio:

Matakai		Zazzabi	Lokaci	Zagaye
1	Pre-denaturation	95 ℃	2 min	1
2	Denaturation	95 ℃	10 s ku	45
2	Annealing, tsawo, sayan haske	60 ℃	30s ku	45

Ka'idar BTK-8:

Matakai		Zazzabi	Lokaci	Zagaye
1	Pre-denaturation	95 ℃	2 min	1
2	Denaturation	95 ℃	5 s ku	45
2	Annealing, tsawo, sayan haske	60 ℃	14 s ku	45

5. Binciken Sakamako (Don Allah a koma zuwa Manual User Instrument)

Bayan amsawa, za a adana sakamakon ta atomatik.Danna "Bincike" don yin nazari, kuma kayan aikin za su fassara ƙimar Ct ta kowane samfurin a cikin ginshiƙin sakamako ta atomatik.Sakamakon sarrafawa mara kyau da tabbatacce zai dace da masu zuwa "6. Control Control ".

6. Kula da inganci

6.1 Sarrafa mara kyau: Babu Ct ko Ct>40 a cikin tashar FAM, Ct≤40 a cikin tashar CY5 tare da yanayin haɓakawa na yau da kullun.

6.2 Kyakkyawan Sarrafa: Ct≤35 a cikin tashar FAM tare da yanayin haɓakawa na yau da kullun, Ct≤40 a cikin tashar CY5 tare da haɓakar haɓakawa ta al'ada.

6.3 Sakamakon yana aiki idan duk abubuwan da ke sama sun cika.In ba haka ba, sakamakon ba shi da inganci.

Tafsirin sakamako

Ana iya samun sakamako masu zuwa:

	Ct darajar tashar FAM	Ct darajar tashar CY5	Tafsiri
1#	Babu Ct ko Ct> 40	≤40	Kwayar cutar ta Monkeypox mara kyau
2#	≤40	Duk wani sakamako	Kwayar cutar ta Monkeypox tabbatacce
3#	40 zuwa 45	≤40	Sake gwadawa;idan har yanzu yana da 40 ~ 45, bayar da rahoto azaman 1 #
4#	Babu Ct ko Ct> 40	Babu Ct ko Ct> 40	Ba daidai ba

NOTE: Idan sakamakon mara inganci ya faru, ana buƙatar tattara samfurin kuma a sake gwadawa.

Bayanin oda

Sunan samfur	Cat.A'a	Girman	Misali	Rayuwar Rayuwa	Trans.& Sto.Temp.
Kwayar cutar Monkeypox na Real Time PCR Kit	Saukewa: B001P-01	48 gwaje-gwaje/kit	Serum/ Lesion Exudate	Watanni 12	-25 ～ -15 ℃