Maganar Prokaryotic Protein
An yarda da tsarin maganganun prokaryotic E. coli a matsayin tsari mai tsada sosai, balagagge, kuma tsarin da aka saba amfani dashi don bayyana furotin.A Bioantibody, mun himmatu don samarwa abokan cinikinmu cikakkiyar sabis na tsayawa ɗaya daga haɗawar kwayoyin halitta zuwa bayanin furotin da tsarkakewa.Ayyukanmu sun haɗa da haɓaka codon kyauta da kuma amfani da fasahar mallakarmu don magance duk wani al'amurran da suka shafi ƙananan magana da rashin daidaituwa da za su iya tasowa yayin duk aikin magana da tsarkakewa.Abokan cinikinmu kawai suna buƙatar samar da jerin kwayoyin halitta ko amino acid na furotin kuma za mu iya isar da furotin mai inganci cikin sauri kamar makonni uku.Bugu da ƙari, Bioantibody yana ba da cirewar endotoxin da sabis na sanya alama wanda ya dace da takamaiman bukatun abokan cinikinmu.Mun himmatu wajen samar da sakamako kuma mun yi alƙawarin ba za mu caje kowane kuɗi ba idan ba a bayyana furotin na ƙarshe ba.
Abubuwan Sabis | Abun Gwaji | Lokacin Jagora (BD) |
Gene Synthesis | Ƙaddamar da Codon, haɓakar kwayoyin halitta da subcloning. | 5-10 |
Ƙididdigar Bayyanawa da Nazarin Solubility | 1. Canji da ƙaddamarwa, ganowar magana tare da SDS-PAGE.2. Binciken Solubility, SDS-PAGE da gano WB | 10 |
Babban shiryawa da tsarkakewa, furotin na ƙarshe (tsarki> 85%, 90%, 95%) da daidaitaccen rahoton gwaji | Tsarkakewar kusanci (Ni shafi, MBP, GST) |
Idan kwayar halitta ta hada a cikiBioantibody, plasmid da aka gina za a haɗa shi a cikin abubuwan da ake bayarwa.