Amfani da Niyya:
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) gwaji ne na in vitro, mai sauri, gwajin kwarara na gefe, wanda kuma aka sani da gwajin immunochromatographic na gefe, wanda aka yi niyya don gano ƙimar S. Typhi da antigens Paratyphi a cikin samfuran fecal daga marasa lafiya.Sakamako daga S. Typhi/Paratyphi Combo Antigen Rapid Test Kit yakamata a fassara shi tare da kimantawar asibiti na majiyyaci da sauran hanyoyin bincike.
Ka'idodin Gwaji:
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit(Immunochromatographic Assay) wani immunoassay na chromatographic na gefe ne.Yana da layukan da aka riga aka rufa da su guda uku, “T1” S. Layin gwajin Typhi, “T2” Layin gwajin Paratyphi da “C” Layin Sarrafa akan membrane na nitrocellulose.Mouse monoclonal anti-S.An rufe magungunan typhi da anti-Paratyphi a kan yankin gwajin gwajin kuma an rufe goat anti-chicken IgY antibodies akan yankin sarrafawa.Lokacin da aka sarrafa samfurin kuma an kara da shi a cikin samfurin da kyau, S. Typhi / Paratyphi antigens a cikin samfurin suna hulɗa da su. da S. Typhi/Paratyphi Antibody-labeled conjugate kafa antigen-antibody launi barbashi hadaddun.Rukunin suna yin ƙaura a kan membrane na nitrocellulose ta hanyar aikin capillary har zuwa layin gwaji, inda linzamin linzamin kwamfuta monoclonal anti-S ya kama su.Kwayoyin rigakafin typhi/Paratyphi.Ana iya ganin layin T1 mai launi a cikin taga sakamakon idan S. Typhi antigens suna cikin samfurin kuma ƙarfin ya dogara da adadin S. Typhi antigen.Ana iya ganin layin T2 mai launi a cikin taga sakamakon idan Paratyphi antigens suna cikin samfurin kuma ƙarfin ya dogara da adadin Paratyphi antigen.Lokacin da S.Typhi/Paratyphi antigens a cikin samfurin ba ya wanzu ko kuma yana ƙasa da iyakar ganowa, babu wata ƙungiya mai launin gani a cikin layin Gwaji (T1 da T2) na na'urar.Wannan yana nuna mummunan sakamako.Babu layin gwaji ko layin sarrafawa a cikin taga sakamakon kafin amfani da samfurin.Ana buƙatar layin sarrafawa na bayyane don nuna sakamakon yana aiki
Bangaren REF REF | B033C-01 | B033C-05 | B033C-25 |
Gwaji Cassette | 1 gwaji | 5 gwaje-gwaje | 25 gwaje-gwaje |
Buffer | kwalba 1 | kwalba 5 | 25/2 kwalabe |
Jakar jigilar kayayyaki | guda 1 | 5 guda | 25 guda |
Umarnin don Amfani | guda 1 | 5 guda | 25 guda |
Certificate of Conformity | guda 1 | guda 1 | guda 1 |
Mataki 1: Samfure Shiri
1. Tattara samfuran najasa a cikin kwantena masu tsabta, masu hana zubewa.
2. Samfuran Sufuri da Ajiye: Ana iya adana samfuran a cikin daki na zafin jiki na awanni 8 ko a sanyaya su a 36°F zuwa 46°F (2°C zuwa 8°C) har zuwa awanni 96.
3. Samfuran najasa waɗanda aka adana a daskarewa na iya narke har sau 2 a -10°C ko ƙasa.Idan ana amfani da samfuran daskararre, narke a zafin jiki.Kar a ƙyale samfuran najasar ta kasance a cikin cakuda mai narkewa na> 2 hours.
Mataki na 2: Gwaji
1. Da fatan za a karanta umarnin a hankali kafin gwaji.Kafin gwaji, ba da damar kaset ɗin gwajin, samfurin samfurin da samfuran su daidaita zuwa zafin jiki (15-30 ℃ ko 59-86 digiri Fahrenheit).
2. Cire kaset ɗin gwaji daga jakar jakar ka sanya a kan shimfidar wuri.
3.Unscrew kwalban samfurin, yi amfani da sandar mai amfani da aka haɗe da aka haɗe a kan hula don canja wurin ƙananan samfurin stool (3- 5 mm a diamita; kimanin 30-50 MG) a cikin kwalban samfurin da ke dauke da samfurin shirye-shirye.
4. Sauya sandar a cikin kwalabe kuma ƙara ƙarfafawa.Mix samfurin stool tare da buffer sosai ta hanyar girgiza kwalban sau da yawa kuma barin bututu shi kadai na minti 2.
5. Cire samfurin kwalban samfurin kuma riƙe kwalban a tsaye a kan rijiyar samfurin Cassette, sadar da 3 saukad da (100 -120μL) na diluted stool samfurin zuwa samfurin da kyau.
Mataki na 3: Karatu
Karanta sakamakon a cikin mintuna 15-20.Lokacin bayanin sakamakon bai wuce mintuna 20 ba
1. S. Typhi Kyakkyawan Sakamako
Makada masu launi suna bayyana a duka layin gwaji (T1) da layin sarrafawa (C).Yana nuna sakamako mai kyau ga S. Typhi antigens a cikin samfurin.
2. Paratyphi Kyakkyawan sakamako
Makada masu launi suna bayyana a duka layin gwaji (T2) da layin sarrafawa (C).Yana nuna sakamako mai kyau ga paratyphi antigens a cikin samfurin.
3. S. Typhi da Paratyphi Kyakkyawan sakamako
Makada masu launi suna bayyana a duka layin gwaji (T1), layin gwaji (T2) da layin sarrafawa (C).Yana nuna sakamako mai kyau ga S. Typhi da Paratyphi antigens a cikin samfurin.
4. Sakamako mara kyau
Ƙungiya masu launi suna bayyana a layin sarrafawa (C) kawai.Yana nuna cewa ƙaddamarwar S. Typhi ko Paratyphi antigens ba su wanzu ko ƙasa da iyakar gano gwajin.
5. Sakamako mara inganci
Babu bandeji mai launin gani da ke bayyana a layin sarrafawa bayan yin gwajin.Wataƙila ba a bi umarnin daidai ba ko gwajin ya lalace.Ana ba da shawarar cewa a sake gwada samfurin
Sunan samfur | Cat.A'a | Girman | Misali | Rayuwar Rayuwa | Trans.& Sto.Temp. |
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit(Immunochromatographic Assay) | B033C-01 | 1 gwaji/kit | Fecal | Watanni 24 | 2-30 ℃ |
B033C-05 | 5 gwaje-gwaje/kit | ||||
B033C-25 | 25 gwaje-gwaje/kit |