Kit ɗin Gwajin Saurin Syphilis (Chromatography na Lateral)

Cikakken Bayani:

Amfani da Niyya:

Kit ɗin Gwajin Saurin Syphilis (Lateral Chromatography) shine saurin immunoassay na chromatographic don gano ingantattun ƙwayoyin rigakafin TP a cikin jini gabaɗaya, ruwan magani ko plasma don taimakawa a gano cutar syphilis.

Ka'idodin Gwaji:

Kit ɗin gwajin sauri na Syphilis ya dogara ne akan gwajin immunochromatographic don gano ƙwayoyin rigakafin TP a cikin jini gaba ɗaya, jini ko plasma.Yayin gwajin, ƙwayoyin rigakafi na TP suna haɗuwa tare da TP antigens da aka lakafta akan barbashi masu launi don samar da hadaddun rigakafi.Sakamakon aikin capillary, hadadden rigakafi yana gudana a cikin membrane.Idan samfurin ya ƙunshi ƙwayoyin rigakafi na TP, za a kama shi ta wurin gwajin da aka riga aka yi masa rufi kuma ya samar da layin gwaji na bayyane.Don zama mai sarrafa hanya, layin sarrafawa mai launi zai bayyana idan an yi gwajin da kyau

Babban Abubuwan Ciki:

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Don Cassette:

Gudun Ayyuka

• Mataki 1: Samfura Shiri

Za'a iya yin Kit ɗin Gwajin Sauri na Syphilis (Lateral Chromatography) ta amfani da jini gabaɗaya, jini ko jini.

1. Raba ruwan jini ko plasma daga jini da wuri-wuri don guje wa hemolysis.Yi amfani da kawai bayyanannun samfuran marasa hemolyzed.

2. Ya kamata a yi gwaji nan da nan bayan an tattara samfuran.Idan ba za a iya kammala gwajin nan da nan ba, ya kamata a adana samfurin jini da plasma a zazzabi na 2-8 ° C har zuwa kwanaki 3, don adana dogon lokaci, samfuran samfuran yakamata a adana su a -20 ℃.Dukkan jinin da aka tara ta hanyar venipuncture yakamata a adana shi a zazzabi na 2-8 ° C idan za a gudanar da gwajin a cikin kwanaki 2 na tarin.Kada a daskare duka samfuran jini.Dukkan jinin da aka tattara ta hannun yatsa yakamata a gwada shi nan da nan.

3. Dole ne a dawo da samfurori zuwa zafin jiki kafin gwaji.Ana buƙatar samfuran daskararre a narke gaba ɗaya kuma a gauraye su sosai kafin gwaji, guje wa daskarewa akai-akai da narke.

4. Idan za a aika samfurori, ya kamata a cika su bisa ga ka'idodin gida da suka shafi jigilar magunguna.

• Mataki na 2: Gwaji

Bada damar tsiri/kaset ɗin gwajin, samfuri, samfurin diluent don isa daki

zafin jiki (15-30 ° C) kafin gwaji.

1. Cire tsiri/kaset ɗin gwajin daga jakar da aka rufe kuma amfani dashi cikin mintuna 30.

2. Sanya tsiri/kaset ɗin gwajin a kan tsaftataccen wuri mai ma'auni.

2.1 Don samfuran jini ko Plasma:

Riƙe digo a tsaye, zana samfurin har zuwa ƙananan Layin Cika (kimanin 40uL), sannan a canja wurin samfurin zuwa samfurin da kyau (S) na telin gwaji/kaset, sannan ƙara digo 1 na samfurin diluent (kimanin 40uL) sannan a fara. mai lokaci.A guji tarko kumfa a cikin rijiyar samfurin (S).Dubi hoton da ke ƙasa.

2.2 Don Gabaɗayan Jini (Venipuncture/Fingerstick) Samfura:

Rike digo a tsaye, zana samfurin zuwa babban Layin Cika (kimanin 80uL), sannan a tura jini gaba ɗaya zuwa samfurin da kyau (S) na telin gwaji/kaset, sannan ƙara digo 1 na diluent samfurin (kimanin 40uL) sannan fara mai lokaci.A guji tarko kumfa a cikin rijiyar samfurin (S).Dubi hoton da ke ƙasa.

• Mataki na 3: Karatu

3. Karanta sakamakon a gani bayan mintuna 10-20.Sakamakon ba shi da inganci bayan mintuna 20.

Fassarar sakamako

1. Sakamako Mai Kyau

Idan duka layin kula da ingancin C da layin gano T sun bayyana, yana nuna cewa samfurin ya ƙunshi adadin ƙwayoyin rigakafi na TP, kuma sakamakon yana da kyau ga syphilis.

2. Sakamako mara kyau

Idan kawai layin C mai inganci ya bayyana kuma layin T ɗin bai nuna launi ba, yana nuna cewa ba a iya gano ƙwayoyin rigakafin TP a cikin samfurin.kuma sakamakon ba shi da kyau ga syphilis.

3. Sakamako mara inganci

Babu bandeji mai launin gani da ke bayyana a layin sarrafawa bayan yin gwajin, sakamakon gwajin ba shi da inganci.Sake gwada samfurin.

Bayanin oda: