Maganar Sunadaran Kwayoyin Yisti
Tsarin furcin yisti hanya ce da ake amfani da ita don bayyana furotin eukaryotic, saboda sauƙin sa a cikin noma, araha, da sauƙin aiki.Daga cikin nau'o'in yisti iri-iri, Pichia pastoris shine mashahurin mai masaukin baki, saboda yana sauƙaƙe maganganun furotin na ciki da na waje.Hakanan tsarin yana ba da damar gyare-gyaren bayan fassarorin, kamar phosphorylation da glycosylation, yana haifar da keɓaɓɓen tsarin magana na eukaryotic tare da fa'idodi masu yawa.
Abubuwan Sabis | Lokacin Jagora (BD) |
Ƙaddamar da Codon, haɓakar kwayoyin halitta da subcloning | 5-10 |
Klone mai inganci | 10-15 |
Ƙananan magana | |
Babban sikelin (200ML) magana da tsarkakewa, abubuwan da za a iya bayarwa sun haɗa da ingantaccen furotin da rahoton gwaji |
Idan an haɗa kwayar halittar a cikin Bioantibody, plasmid ɗin da aka gina za a haɗa shi cikin abubuwan da ake iya bayarwa.