Amfani da Niyya
Wannan samfurin ya dace da gwajin gwaji na asibiti na maganin jini/plasma/dukkan samfuran jini don gano ƙwayoyin rigakafin cutar Leishmania.Gwaji ne mai sauƙi, mai sauri da marasa kayan aiki don gano cutar kala-azar da Leishmania ke haifarwa.
Ƙa'idar Gwaji
Wannan samfurin immunoassay ne na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) pad mai launi mai launin burgundy mai ɗauke da recombinant rK39 antigen conjugated da colloid zinariya (Leishmania conjugates) da zomo IgG-gold conjugates;2) nitrocellulose membrane tsiri dauke da nau'ikan gwaji guda biyu (M da G bands) da kuma bandungiyar sarrafawa (C band).
Kayayyakin / bayarwa | Yawan (Gwaji 1/Kit) | Yawan (Gwaji 5/Kit)
| Yawan (Gwaji 25/Kit)
|
Gwajin Kit | 1 gwaji | 5 gwaje-gwaje | 25 gwaje-gwaje |
Buffer | kwalba 1 | kwalabe 5 | 25/2 kwalabe |
Dropper | guda 1 | 5 guda | 25 guda |
Jakar jigilar kayayyaki | guda 1 | 5 guda | 25 guda |
Lancet mai zubarwa | guda 1 | 5 guda | 25 guda |
Umarnin Don Amfani | guda 1 | guda 1 | guda 1 |
Certificate of Conformity | guda 1 | guda 1 | guda 1 |
Tattara Serum/Plasma/Jini duka daidai gwargwado.
Da fatan za a karanta umarnin a hankali kafin gwaji.Kafin gwaji, ba da damar kayan gwajin, maganin samfurin da samfurin su daidaita zuwa zafin jiki (15-30 ℃ ko 59-86 digiri Fahrenheit).
1.Cire bututun cirewa daga kit da akwatin gwaji daga jakar fim ta yayyage daraja.Saka su a kan jirgin sama a kwance.
2.Bude katin dubawa aluminum jakar jakar.Cire katin gwajin kuma sanya shi a kwance akan tebur.
3.Yi amfani da pipette mai zubarwa, canja wurin digo ɗaya (kimanin 20μL) jinin yatsa / ko 4μL serum / ko 4μL plasma/ ko 4μL duka jini a cikin samfurin da kyau akan kaset ɗin gwaji.
4.Bude buffer buffer.Saka digo 3 (kimanin 80 μL) na diluent na gwaji a cikin diluent mai siffa mai kyau mai zagaye.Fara kirgawa.
Karanta sakamakon a 5-10 mins.Sakamako bayan mintuna 10 ba su da inganci.
Sakamakon mara kyau
Idan kawai layin kula da ingancin C ya bayyana kuma layin gano G da M basa nunawa
Sakamako Mai Kyau
1.Dukansu layin kula da ingancin C da layin ganowa M sun bayyana = an gano antibody Leishmania IgM, kuma sakamakon yana da kyau ga IgM antibody.
2.Dukansu layin kula da ingancin ingancin C da layin ganowa G sun bayyana = an gano Leishmania IgG antibody kuma sakamakon yana da kyau ga rigakafin IgG.
3.Dukansu layin kula da ingancin C da layin ganowa G da M sun bayyana = ƙwayoyin rigakafin Leishmania IgG da IgM.An gano, kuma sakamakon yana da kyau ga duka IgG da IgM antibodies.
Sakamakon mara inganci
Ba za a iya lura da layin sarrafa ingancin C ba, sakamakon ba zai yi aiki ba ko da kuwa layin gwaji ya nuna, kuma yakamata a maimaita gwajin.
Sunan samfur | Cat.A'a | Girman | Misali | Rayuwar Rayuwa | Trans.& Sto.Temp. |
Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay) | B020C-01 | 1 gwaji/kit | Magani/Plasma/Jini Gabaɗaya | Watanni 18 | 2-30 ℃ / 36-86 ℉ |
B020C-05 | 5 gwaje-gwaje/kit | ||||
B020C-25 | 25 gwaje-gwaje/kit |